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Evaluation of Genetic Resources from Global Collections of Cultivated Diploid Cotton

Principal Investigator: Randy D. Allen

Objectives:

Our long-term goal is to identify novel molecular markers that are linked to important quantitative traits in G. arboreum L. (A2) and G. herbaceum L. (A1). The simpler genetic composition of these diploids may facilitate the identification of markers that are closely linked to genes that determine important traits. We anticipate that alleles for these genes may differ from those in cultivated upland cotton (G. hirsutum L. [AADD]) and may be exploited through introgression from diploid species. Also, the genetic linkage between molecular markers and important genes that we identify in A genome cottons is likely to be conserved in other species, including upland cotton. These orthologous relationships will allow for screening of diverse cotton germplasm for novel alleles that can be introgressed into breeding lines.

Specific Objectives:

  • Isolate candidate genes from G. herbaceum and G. arboreum that are likely to be involved in determining fiber quality, stress tolerance and other quantitative traits.
  • Bioinformatic comparison of sequences of these A1 and A2 orthologs to identify potential single nucleotide polymorphisms (SNPs) and insertion/deletions (InDels) for use in genetic mapping.
  • Molecular confirmation of SNPs and development of detection assays for rapid screening of these genetic markers in mapping populations.

Heritability of Fiber Length Distribution

Principal Investigators: Eric Hequet, Wayne Smith

Co-Worker: Chris Braden

Objectives:

The ITC conducted research on the effect of length distribution on yarn quality. It showed a marked effect of both the shortest and longest fibers on yarn quality. Since length distributions clearly differ among varieties, it may provide a new tool for cotton breeders in their efforts to improve fiber length characteristics.

Specific Objectives:

  • Determine whether genes present in three near-long staple (NLS) upland cotton genotypes are allelic
  • Determined transgressive segregation potential for NLS fiber in segregating populations developed with NLS upland parents
  • Measuring the heritability of fiber properties with the AFIS instrument

Increasing the Marketing Value of Cottonseed as a Pre-Harvest Control Measure for E. coli O157:H7 in Feedlot Cattle

Principal Investigators: Mindy Brashears, Michael Galyean

Objectives:

The objective of this study is to determine the effects of feeding whole cottonseed to feedlot steers near the end of the feeding period on the shedding of E. coli O157:H7. Feedlot performance for typical feeding period and carcass quality will also be measured to ensure that these variables are not compromised by feeding whole cottonseed.