Amanda M.V. Brown
- Postdoctoral Fellow (AAUW)/Postdoc Scholar (Comparative Genomics), Oregon State University, Department of Integrative Biology
- Postdoctoral Research Assistant (Population Genomics), University of Montana, Division of Biological Sciences
- Ph.D. in Molecular Evolution, University of British Columbia, Department of Zoology
- B.Sc. Honours (Ecology), University of British Columbia
Dr. Brown's lab explores how innovation emerges from microbial symbiosis, using high
throughput genome and transcriptome sequencing, bioinformatics, population and comparative
genomics, confocal microscopy, phylogenomics, and microevolutionary analysis.
The lab examines several systems, with a focus on agriculture and disease: (1) bacteria in plant-parasitic nematodes (2) bacteria in hemipteran insects (3) multi-trophic communities in the rhizosphere (involving plants, plant-pests, fungi, viruses, and bacteria) (4) beneficial microbes associated with plant and tree leaves, and (5) other microbes associated with humans and mosquitos.
This work centers on “big data" analysis, and includes shotgun metagenomics, de novo assembly, annotation, comparative pathway enrichment analysis, and detection of selection. Her research also extends to software development to implement algorithms to aid in biological discovery from these complex datasets.
Microbes are ubiquitous, occurring in every habitat on earth. Stable, mutually beneficial interactions have evolved in all major groups across the tree of life. Microbe-host interactions have evolved to serve biochemically fundamental processes independently many times. Examples include organelles such as mitochondria and chloroplasts, obligate intracellular endosymbionts such as bacteria within insect bacteriomes, and beneficial microbes that make up the human microbiome.
Despite the importance of host-associated microbes, key questions remain unanswered. Using cutting-edge techniques, Dr. Brown’s lab investigates the following questions:
Origins? How do microbial partnerships originate and what is the role of horizontal gene transfer?
Maintenance? How are microbial partnerships maintained in the face of evolutionary and ecological pressure? How do host and microbe regulate their biochemical interactions?
Disruption? How can disruption of these partnerships lead to species invasions, or disease outbreaks? What do models predict about stability of microbial mutualism?
Complexity? What are the costs of complexity and opposing selection at different levels, and how do hosts and microbes overcome this?
Impact? What role do microbial partnerships have in the evolution of adaptive innovation, disease resistance, biomass production, and ecosystem stability?
2016 Brown AMV, Wasala SK, Howe DK, Peetz AB, Zasada IA, Denver DR. Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia hosts. Scientific Reports 6: 34955. doi:10.1038/srep34955
2016 Phillips WS, Brown AMV, Howe DK, Peetz AB, Blok VC, Denver DR, Zasada IA. The mitochondrial genome of Globodera ellingtonae is composed of two circles with segregated gene content and differential copy numbers. BMC Genomics 17:706. doi:10.1186/s12864-016-3047-x
2016 Denver DR, Brown AMV, Howe DK, Peetz AB, Zasada IA Genome skimming: a rapid approach to gaining diverse biological insights into multicellular pathogens. PLoS Pathogens 12(8): e1005713. doi:10.1371/journal.ppat.1005713
2015 Brown AMV, Howe DK, Peetz AB, Wasala SK, Zasada IA, Denver DR. Comparative genomics of an endosymbiont in a plant-parasitic nematode suggest a role in nutritional symbiosis.Genome Biology & Evolution 7(9):2727-2746. doi:10.1093/gbe/evv176
2014 Brown AMV, Huynh LY, Bolender CM, Nelson KG, McCutcheon JP. Population genomics of a symbiont in the early stages of a pest invasion. (Invited original research article for Nature's Microbiome). Molecular Ecology 23:1516-1530. doi: 10.1111/mec.12366
2014 Brown AMV. Microevolution of insect-bacterial mutualists: a population genomics perspective. In Evolutionary Biology: Genome Evolution, Speciation, Coevolution and Origin of Life. Pontarotti, P. (ed.). Springer International, Switzerland p. 247-259.
2010 Brown AMV, Kent ML, Adamson ML. Description of five new Loma (Microsporidia) species in pacific fishes with redesignation of the type species Loma morhua Morrison & Sprague, 1981, based on morphological and molecular species-boundaries tests. Journal of Eukaryotic Microbiology 57(6): 529-553. doi:10.1111/j.1550-7408.2010.00508.x
2010 Brown AMV, Kent ML, Adamson ML. Low genetic variation in the salmon and trout parasite Loma salmonae (Microsporidia) supports marine transmission and clarifies species boundaries. Diseases of Aquatic Organisms 91(1): 35-46. doi:10.3354/dao02246
2008 Lee R, Williams BAP, Brown AMV, Adamson ML, Keeling PJ, Fast NM. Alpha and beta-tubulin phylogenies support a close relationship between the microsporidia Brachiola algerae and Antonospora locustae.Journal of Eukaryotic Microbiology 55(5): 388-392.
2006 Brown AMV, Adamson ML. Phylogenetic distance of Thelohania butleri Johnston, Vernick and Sprague, 1978 (Microsporidia; Thelohaniidae), a parasite of the smooth pink shrimp Pandalus jordani, from its congeners suggests need for major revision of the genus Thelohania Henneguy, 1892. Journal of Eukaryotic Microbiology 53(6): 445-455.
2002 Brown, Amanda MV, Coury, Michael. REALEM: Realistic Evolutionary Models for DNA Analysis. Version 1.0. Computer Software Package in Java 1.3.1, distributed by authors.
2002 Brown AMV, Kent ML. Molecular diagnostics for Loma salmonae and Nucleospora salmonis (microsporidia) In Molecular diagnostics of salmonid diseases. Cunningham, C. O. (ed.). Kluwer Academic Publishers, Dordrecht p. 267-283.
2016 Brown AMV, Howe DK, Wasala SK, Peetz AB, Zasada IA, Denver DR. Evolutionary genomics of an ancestral Wolbachia from plant-parasitic nematodes. Jun 28-Jul 3, Lamington Park, Queensland, Australia.
2016 Brown AMV, Howe DK, Wasala SK, Peetz AB, Zasada IA, Denver DR. Evolutionary genomics of rhizosphere endosymbionts: getting to the root of it. Yosemite Symbiosis Workshop, May 6-8. Wawona, Yosemite National Park, CA.
2015 Invited speaker Brown AMV, Howe DK, Peetz AB, Phillips WS, Zasada IA, Denver DR. Genome skimming: novel insights into plant-parasitic nematode genomics and potential avenues for management. Symposium: Building Applied and Genomic Nematology Partnerships, Society of Nematologists Meetings, Jul 19-24, 2015, East Lansing, MI.
2015 Brown AMV, Dettwyler L, Wernick RI, Denver DR. DeNAngler: a DNA untangling algorithm for mixed strain de novo assembly. Spring Conference of the Center for Genomics and Biocomputing, Oregon State University.
2014 Invited speaker Brown AMV, Huynh LY, McCutcheon JP. Population genomic and transcriptomic insights into symbiont adaptation during a rapid insect invasion. Horizons in the Field of Symbiosis Member Symposium, Entomological Society of America Meetings, Nov 15-19, 2014, Portland, OR.
2013 Invited speaker Brown AMV, McCutcheon JP. Microevolution of insect-bacterial mutualists: a population genomics perspective. 17th Evolutionary Biology Meeting at Marseilles, September 17-20, 2013, Marseille, France.
2008 Invited speaker Brown AMV. Pondering parasitism: meaning out of madness in the microsporidia. Invited talk to faculty at the Hawaiian Institute of Marine Biology (HIMB), University of Hawaii.
2008 Invited speaker Brown AMV. Meaning out of madness in the microsporidia. Invited talk to the Integrative Graduate Education and Research Traineeship (IGERT) group, University of Hawaii.