Texas Tech University

Peter Keyel

Assistant Professor

Email: peter.keyel@ttu.edu

Phone: 1(806)834-6248

  • PhD University of Pittsburgh, 2006
  • BS University of Minnesota Duluth 2001

Web Links:

Research Interests

Inflammation: macrophage responses to pore-forming toxins

Inflammation is a crucial component of many diseases, regardless of whether they are caused by autoimmunity, pathogenic microbes, tumors, or even particles and chemicals. Inflammation is also necessary to develop robust responses to vaccines. This inflammation depends on the response of macrophages and other innate immune cells to pathogenic conditions.

The research in my lab aims to elucidate novel mechanisms of inflammation by studying the interplay and downstream effects of bacterial pore-forming toxins, which trigger inflammation, on macrophages. Streptolysin O is an archetypal pore-forming toxin that can directly lyse cells. Interestingly, macrophages can withstand high doses of toxin. At these sublytic concentrations, macrophages resist the damage and execute a pro-inflammatory pathway. Understanding what mechanisms are required for damage resistance will help us regulate the inflammatory response, as well as apply that knowledge to other diseases directly related to membrane damage, such as muscular dystrophies.

A second aspect of the inflammatory response is the mechanism through which macrophages alert the rest of the immune system and elaborate the inflammatory response. To accomplish this, macrophages utilize a complex called the inflammasome to promote cytokine processing and release. In addition to  cytokine processing and release, the macropahges also commit suicide via the pyroptotic cell death pathway. Elucidating how macrophages switch from incredible resistance to membrane damage induced by SLO and other agents to dismantling this resistance and tearing their own membranes apart will help us understand the fundamental cell death and repair mechanisms that lie at the heart of inflammation.

Selected Publications

  • Current, complete list of publications

  • Romero, M., Keyel, M., Shi, G., Bhattacharjee, P., Roth, R., Heuser, J.E., and P.A. Keyel. (2017). Intrinsic repair protects cells from pore-forming toxins by microvesicle shedding. Cell Death Differ, 24(5):798-808.

  • Shi, G., Abbott, K.N., Wu, W., Salter, R.D., and P.A. Keyel. (2017). Dnase1L3 Regulates Inflammasome-Dependent Cytokine Secretion. Front. Immunol. 8:522

  • Keyel, P.A., Roth, R., Yokoyama, W.M., Heuser, J.E. and R.D. Salter. (2013) Reduction of SLO Pore-forming Activity Enhances Inflammasome Activation. Toxins (Basel), 5(6):1105-1118. PMCID: PMC3717772.
  • Keyel, P.A., Heid, M.E., Watkins, S.C. and R.D. Salter. (2012) Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy. J Vis Exp, Oct 1(68): 4227.
  • Keyel, P.A., Tkacheva, O.A., Larregina, A.T. and R.D. Salter. (2012) Coordinate Stimulation of Macrophages by Microparticles and TLR Ligands Induces Foam Cell Formation. J Immunol, 189(9):4621-4629. PMCID: PMC3478499
  • Keyel, P.A., Loultcheva, L., Roth, R., Salter, R.D., Watkins, S.C., Yokoyama, W.M. and J.E. Heuser. (2011) Streptolysin O Clearance Via Sequestration Into Blebs That Bud Passively From the Plasma Membrane. J. Cell Sci., 124(14): 2414-2423. PMCID: PMC3124372
  • Cooper, M.A., Elliott, J.M., Keyel, P.A., Yang, L., Carrero, J.A., and W.M. Yokoyama. (2009) Cytokine-Induced Memory-Like Natural Killer Cells. PNAS, 106(6):1915-1919. PMCID: PMC2644138
  • Keyel, P.A., Thieman, J.R., Roth, R., Erkan, E., Everett, E.T., Watkins, S.C., Heuser, J.H., and L.M. Traub. (2008) The AP-2 Adaptor Beta2 Appendage Scaffolds Alternate Cargo Endocytosis. Mol Biol. Cell., 19(12): 5309-2326. PMCID: PMC2592659
  • Tripathy, S.K., Keyel, P.A., Yang, L., Pingel, J.T., Cheng, T.P., Schneeberger, A. and W.M. Yokoyama. (2008) Continuous Engagement of a Self-MHC-specific Activation Receptors Induces NK Cell Tolerance. J. Exp. Med., 205(8): 1829-1841. PMCID: PMC2525593

Department of Biological Sciences

  • Address

    Department of Biological Sciences, Texas Tech University, Box 43131 Lubbock, TX 79409
  • Phone

    806.742.2715
  • Email

    biology@ttu.edu