Fee Schedule
Custom oligonucleotides through IDT (Integrated DNA Technologies, Inc)
*25 nmol scale synthesis (basic) $0.25/base
*Many other synthesis scales are available as well as various modifications for a higher price.
*Desalted custom synthesized DNA oligos come lyophilized with a yield guarantee.
*The common primers listed below are in stock and available immediately. $15.00/10 ug
(cleaved, deprotected, quantified and lyophilized)
| 5' 3' | |
| M13 17-mer Universal Primer (-20) | GTA AAA CGA CGG CCA GT |
| M13 16-mer Reverse Sequencing Prime | AAC AGC TAT GAC CAT G |
| Lambda gt11 Sequencing primer (forward) | GGT GGC GAC GAC TCC TGG AGC CCG |
| Lambda gt11 Sequencing primer (reverse) | TTG ACA CCA GAC CAA CTG GTA ATG |
| T7 Promoter primer | TAA TAC GAC TCA CTA TAG GG |
| T3 Promoter Sequencing primer | ATT AAC CCT CAC TAA AGG GA |
| Sp6 Promoter Sequencing primer | CGA TTT AGG TGA CAC TAT AG |
DNA SEQUENCING
ABI 3130 4-capillary $7.50 per reaction
*Includes sequencing reaction and capillary electrophoretic sequencing run.
*User supplies template DNA (typically at 100ng/ul water) and any custom primer (typically at 1pmol/ul water)
*Approximately 2 pmol primer is required per sequencing reaction.
*Direct PCR fragment sequencing requires 10ng per 100bp of template fragment.
*Plasmid sequencing requires at least 300ng template DNA per reaction.
*Phage sequencing requires >500ng template DNA per reaction, so it is usually submitted at a higher concentration.
FRAGMENT ANALYSIS
CEQ 8000 8-capillary see staff for requirements and pricing
PROTEIN IDENTIFICATION
Electrospray Mass Spectrometry $30.00 per run
(User should supply 1 pmol of digested protein (typically tryptic digest) and should see staff for further information)
MALDI-TOF/TOF Mass Spectrometry $20.00 per run
(User should see staff for information on sample requirements)
*Tryptic Digest $10.00 per protein
*Zip-tip desalting/purification $5.00
PROTEIN PURIFICATION
Low pressure chromatography *Please see staff for
Medium pressure, rapid chromatography pricing information
2D-gel Electrophoresis
* 7 cm format $30.00
*11 cm format $35.00
*17 cm format $40.00
*In-gel tryptic digest $10.00
APPLIED PCR TECHNIQUES
Real-Time PCR $6.00 per run
*Plates are available to purchase for $6.00 per plate and covers are available for $1.00 per cover.
Other thermocyclers see staff for availability
OTHER INSTRUMENTS AVAILABLE FOR USE:
Biacore X
ELISA Plate Reader
Biomek 2000
*Please see staff for requirements and pricing.
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