Custom oligonucleotides through IDT (Integrated DNA Technologies, Inc)
*25 nmol scale synthesis (basic) $0.25/base
*Many other synthesis scales are available as well as various modifications for a higher price.
*Desalted custom synthesized DNA oligos come lyophilized with a yield guarantee.
*The common primers listed below are in stock and available immediately. $15.00/10 ug
(cleaved, deprotected, quantified and lyophilized)
|M13 17-mer Universal Primer (-20)||GTA AAA CGA CGG CCA GT|
|M13 16-mer Reverse Sequencing Prime||AAC AGC TAT GAC CAT G|
|Lambda gt11 Sequencing primer (forward)||GGT GGC GAC GAC TCC TGG AGC CCG|
|Lambda gt11 Sequencing primer (reverse)||TTG ACA CCA GAC CAA CTG GTA ATG|
|T7 Promoter primer||TAA TAC GAC TCA CTA TAG GG|
|T3 Promoter Sequencing primer||ATT AAC CCT CAC TAA AGG GA|
|Sp6 Promoter Sequencing primer||CGA TTT AGG TGA CAC TAT AG|
ABI 3130 4-capillary $7.50 per reaction
*Includes sequencing reaction and capillary electrophoretic sequencing run.
*User supplies template DNA (typically at 100ng/ul water) and any custom primer (typically at 1pmol/ul water)
*Approximately 2 pmol primer is required per sequencing reaction.
*Direct PCR fragment sequencing requires 10ng per 100bp of template fragment.
*Plasmid sequencing requires at least 300ng template DNA per reaction.
*Phage sequencing requires >500ng template DNA per reaction, so it is usually submitted at a higher concentration.
CEQ 8000 8-capillary see staff for requirements and pricing
Electrospray Mass Spectrometry $30.00-$40.00 per run (consult center personnel)
(User should supply 1 pmol of digested protein (typically tryptic digest) and should see staff for further information)
MALDI-TOF/TOF Mass Spectrometry $30.00 per run
(User should see staff for information on sample requirements)
*Tryptic Digest $10.00 per protein
*Zip-tip desalting/purification $5.00
Low pressure chromatography *Please see staff for
Medium pressure, rapid chromatography pricing information
* 7 cm format $30.00
*11 cm format $35.00
*17 cm format $40.00
*In-gel tryptic digest $10.00
APPLIED PCR TECHNIQUES
Real-Time PCR $6.00 per run
*Plates are available to purchase for $6.00 per plate and covers are available for $1.00 per cover.
Other thermocyclers see staff for availability
OTHER INSTRUMENTS AVAILABLE FOR USE:
ELISA Plate Reader
*Please see staff for requirements and pricing.