Tissue Engineering and Mechanotransduction Lab

Heart valve interactomes: integrin β1 and tryptophan hydroxylase 1 (TPH1)

  • The interactome consists of a molecular network of direct and indirect interactions between proteins in a cell. We are particularly interested in two interactomes:
    • integrin β1
    • serotonin synthesis signaling
  • In order to fully uncover the mechanotransduction mechanisms at the cell membrane, the complete network of interacting proteins in valvular cells needs to be determined. These data will open doors to the regulation of cell adhesion, proliferation and degenerative transformation processes.
  • The goal of this project is two-fold:
    • To confirm predicted interacting partners of protein experimentally
    • To identify novel interacting partners of proteins of interest

integrin and actin
Interaction maps obtained from mass spectrometry data of proteins interacting with α-actin and integrin β1

TPH1
Interaction maps obtained from mass spectrometry data of proteins interacting with TPH1