Nutritional Sciences Research
Resources for Nutritional Sciences Researchers
Centralized Core Facilities:
Texas Tech University provides comprehensive research facilities and resources for biomedical research, with oversight by the Vice President for Research. The Center for Biotechnology and Genomics located in the Experimental Sciences building (ESB) houses state-of-the-art instrumentations and provide services and training in various aspects of modern biotechnology, mass spectrophotometry, biomolecular interaction, and DNA applications including metagenomics, meta-transcriptomics, proteomics, and metabolomics. The facility is fully staffed with personnel to assist with training and research services. Also available is the College of Arts & Sciences Microscopy (CASM), located in the ESB, which offers support and services to scientists with expertise in the fields of optical microscopy, cell cytometry and the preparation of life –science samples for imaging and analysis. The CASM is equipped with automated histology staining system, an advanced bottom-mount camera for the H-8100 transmission electron microscope (TEM) for protein-crystal research, laser capture microdissection microscope, and Olympus Fluoview 1000 Live Cell confocal microscope, to name a few.
Behavioral Medicine Translational Research (BMTR) Laboratory:
Supervised by Dr. Martin Binks, the BMTR lab consists of approximately 3500 square feet of clinical research/mixed use space and 600 square feet of student office areas. It houses 2 Bod Pod devices and 1 BIA machine for the measurement of body composition; a metabolic cart; weight, BP, phlebotomy equipment; centrifuge and computers for data collection (assessment), etc. It has institutionally approved phlebotomy/biohazard certified areas that allow for a wide range of clinical interventions and processing of samples. Our exam and common areas are equipped with bariatric certified/friendly equipment. All equipment and facilities needed to conduct the clinical assessment aspects of the protocol are housed here.
Texas Tech Neuroimaging Institute (TTNI) led by Dr. Eric Walden is located in the Experimental Sciences building at TTU and offers multi-user neuroimaging capabilities. The neuroimaging lab at the Institute houses a Siemens 3T Magnetom Skyra scanner with computing facilities, a mock scanner room, and behavioral testing rooms. The Skyra scanner is equipped for acquiring MRI, fMRI, diffusion tensor imaging (DTI), and MR spectroscopy (MRS) data. A high-density simultaneous EEG/ fMRI system is also available. A dual network server computer is interfaced with the Skyra scanner and the internet for receiving scanner data and providing access of the collected data to the investigators via the internet. The neuroimaging lab also contains three 12-core Mac Pros that are setup for high performance, parallel neuroimaging analysis. In addition, TTNI has access to the linux cluster housed at the High Performance Computing Center (HPCC) at TTU. In addition, the lab offers consultations regarding study design and modification of data acquisition parameters to assistance with data analysis.
Community Nutrition Laboratory:
The community nutrition laboratory is led by Drs. Mary Murimi and Wilna Oldewage-Theron and has the necessary equipment and measuring tools for food and nutrition research such as body composition scales, stadiometers, non-flexible measuring tapes, and food models for dietary intake measurements as well as food preparation equipment for nutrition education demonstrations. The laboratory also has Portable Reflection Spectroscopy Device ("Veggie Meter") for measurements of skin carotenoid levels.
Molecular Biology Laboratories:
Under the directorship of Drs. Naima Moustaïd-Moussa, Shu Wang, Nikhil V. Dhurandhar, and Vijay Hegde, cutting-edge molecular tools are available including:
- Brookhaven BI-MAS and ZetaPALS analyzer system for measuring nanocarrier size, size distribution and zeta potential
- High-Performance Liquid Chromotography (HPLC) that is equipped with Waters1525 binary pump, 2707 autosampler with refrigeration unit, 2489 dual wavelength UV-visible detector, 2475 multi λ fluorescence detector, 2465 electrochemical detector and in-line degasser
- Liquid Chromatography Mass Spectrometry (LC/MS)-2020 UFLC PDA SYSTEM (Shimadzu Corporation) with PDA detector and high-speed single quadrupole LCMS detector with electrospray interface and source, CBM-20A with Switch, DGU-20A5 Degasser, SIL-20AC UFLC Autosampler, two LC-20AD Solvent Delivery Units, semi-micro Flow Cell for SPD-M20A, advanced data station, and compatible LC/MS solution software
- EVoS XL CORE brightfield /phase contrast microscope, auto-fluorescent microscope and inverted miscroscopes for cell and tissue analysis
- LiCor and BioRad ChemiDoc Touch quantitative western imaging system for protein work
- Standard and real-time quantitative PCR instrumentations for DNA/RNA work
- MagPix Luminex instrument for cytokine analysis
- Seahorse XF Extracellular Flux Analyzer for measuring oxygen consumption and energy expenditure in live cells
- Cellometer Vision CBA (Nexcelom) and a BD Flow Cytometer for cellular analysis
- Tri-Carb 4810 TR Liquid Scintillation Analyzer (Perkin Elmer) for measuring radioactive isotopes in serum or plasma samples
Neurobiology / Integrative Physiology Laboratory:
The lab of Dr. Andrew C. Shin is equipped with Stoelting stereotaxic frames for cannulation or direct injection into the brain of rodents for DREADD (designer-receptors-exclusively-activated-by-designer-drugs) studies, optogenetics instrumentations including a waverform generator, energy meter, two lasers, and a beam splitter, and several Harvard single- and multi-syringe automated pumps for integrative physiological pancreatic clamps with tracer dilution technique for metabolic flux and insulin sensitivity analyses. Many other techniques are available in the lab including, but not limited to, venous catheter implantation, sympathectomy, vagotomy, and microdissection of brain.
Texas Tech University has full accreditation by the Council on Accreditation for the
Assessment and Accreditation of Laboratory Animal Care (AAALAC). The College of Human
Sciences building houses an accredited animal facility in its basement, overseen centrally
by Texas Tech Office of Lab Animal Care. This facility includes rooms for animal housing,
surgery/ procedure rooms and includes eight mouse metabolic chambers and a 4-lane
mouse treadmill, all of which are equipped to collect respiratory gases to calculate
TEE, VO2, VCO2, and RER. An EchoMRI-3-in-1/ body composition analyzer for live animals
is also conveniently located in the animal facility. Additionally, a 3600 sq.ft. animal
housing is also available at the Experimental Sciences building on campus which has
ABSLI and ABSLII level animal rooms. This facility contains ante room (with washer/dryer
and locker rooms for men and women), PPE/storage room, 6 animal holding rooms, 3 procedure
rooms, 1 feed/bedding room, cage-wash room (dirty and clean sides) equipped with a
pass-through cage and bottle washer, a pass-through autoclave, a bio-bubble for bedding
disposal and storage for caging, racks, carts, water bottles and enrichment.
Additional equipment available are IVIS spectrum CT machine (anesthesia included), 2 mobile anesthesia machines, 3 hard-ducted biosafety cabinets (Class BII) and 4 recirculating biosafety cabinets (Class Bll). Outstanding staff members are committed to help monitoring the animals seven days a week. Veterinarians are available for consultation if needed.
Institutional Support for Investigators:
Various workshops and seminars related to grant writing and career development are offered on a regular basis to provide support and nurture all faculty members, but especially young investigators into an excellent scientist, a scholar, and a leader. The Obesity Research Cluster (ORC), founded in 2013, aims to develop interdisciplinary basic clinical and community translational research to prevent and treat obesity along with its related complications via using innovative collaborations and strategic partnerships. The ORC offers seminar series with both internal and external experts in nutrition and obesity that provide a platform for research collaborations and training in basic, community and clinical translational research to fight obesity and related comorbidities. In addition, the Office of Research Services offers support for easy research proposal submissions to various agencies (government and non-government) by providing streamlined-internal routing, facilitating proposal submissions to sponsor systems, and by storing proposal and award documents through the use of Cayuse electronic research administration system.