Zemfira Karamysheva, Ph.D.
Phone: 1 (806) 834-5075
- Postdoc, Texas A&M University (2000-2003)
- Postdoc, University of Tokyo (1997-1999)
- Ph.D. Molecular Biology, Moscow State University (1997)
- M.Sc. Biology, Kuban State University (1985)
Regulation of protein translation in parasitic protozoan Leishmania major:Leishmania parasites alternate between promastigotes living in the midgut of sandflies and amastigotes
residing in the mammalian host. The change between hosts involves dramatic temperature,
pH and nutritional stresses. The adaptation to each host and parasite survival in
harsh environment is achieved through differential gene expression. Interestingly,
Leishmania major lacks promoter-mediated regulation of transcription and its gene expression
is predominantly controlled at the level of protein translation and mRNA stability.
We hypothesize that Leishmania ribosomes undergo change in their composition and regulation in order to promote
parasite survival and withstand environmental insults. Through collaboration with
Health Science Center at TTU we use polysome profiling technique to examine translation
and identify players involved in control of Leishmania major translation during different environmental stresses like heat shock, acidic pH and
The role of lipids in regulation of Leishmania gene expression: Lipids play important role in both promastigote and amastigote stages of Leishmaniamajor and many of them are essential for parasite growth and ability to cause pathology. Recently we have found that impairement in sterol synthesis leads to substantially reduced protein and RNA level in the parasite. We seek to determine the role of sterols and other lipids in control of Leishmania gene expression. This will contribute to the understanding of molecular mechanisms of parasite pathogenesis and help to identify new pharmacological targets for further treatments.
Karamysheva, Z. N.*, Gutierrez Guarnizo, S. A., Karamyshev, A. L.* (2020) Regulation of Translation in
the Protozoan Parasite Leishmania, Review, Int. J. Mol. Sci., 21, 2981; doi:10.3390/ijms21082981 (* = corresponding authors).
Karamyshev A. L.*, Tikhonova, E. B., Karamysheva, Z. N.* (2020) Translational Control of Secretory Proteins in Health and Disease. Review, Int. J. Mol. Sci., 21, 2538; doi:10.3390/ijms21072538 (* = corresponding authors).
Karamysheva, Z. N.*, Tikhonova, E. B., Karamyshev *, A. L. (2019) Granulin in Frontotemporal Lobar Degeneration: Molecular Mechanisms of the Disease. Review,Frontiers in Neuroscience, doi: 10.3389/fnins.2019.00395. PMID: 31105517 PMCID: PMC6494926 (* = corresponding authors).
Tikhonova, E. B., Karamysheva, Z. N., von Heijne, G., Karamyshev *, A. L. (2019) Silencing of aberrant secretory protein expression by disease-associated mutations. Journal of Molecular Biology. PMID: 31100385, doi: 10.1016/j.jmb.2019.05.011. PMID: 31100385.
Karamyshev, A. L.*, Karamysheva, Z. N.* Lost in Translation: Ribosome-Associated mRNA and Protein Quality Controls. Review, Front. Genet., 2018, Oct 4;9:431. PMID:30337940 PMCID: PMC6180196 (* = corresponding authors).
Karamysheva, Z. N., Tikhonova, E. B., Grozdanov, P. N., Huffman J. C., Baca, K. R., Karamyshev, A, Denison R. B., MacDonald, C. C., Zhang, K., Karamyshev, A. L. (2018) Polysome Profiling in Leishmania, Human Cells and Mouse Testis. Journal of Visualized Experiments (JoVE), Apr 8; (134) doi: 10.3791/57600.
Dutta AK, Khimji AK, Karamysheva Z, Fujita A, Kresge C, Rockey DC, Feranchak AP (2016) PKCa regulates TMEM16A-mediated Cl-secretion in human biliary cells. Am J Physiol Gastrointest Liver Physiol., 310(1), 34-42.
Karamysheva Z, Díaz-Martínez LA, Warrington R, Yu H. (2015) Graded requirement for the spliceosome in cell cycle progression. Cell Cycle, 14(12), 1873-1883.
Diaz-Martinez L.A., Karamysheva Z.N., Warrington R., Li B., Wei S., Xie X.J., Roth M.G. (2014) Genome-wide siRNA screen reveals coupling between mitotic apoptosis and adaptation. EMBO J., 33(17), 1960-1976.
Karamyshev A.L., Patrick A.E.*, Karamysheva Z.N.*, Griesemer D.S., Hudson H., Tjon-Kon-Sang S., Nilsson I., Otto H., Liu Q., Rospert S., von Heijne G., Johnson A.E., Thomas P.T. (2014) Inefficient SRP Interaction with a Nascent Chain Recruits Ago2 and Triggers Specific mRNA Degradation. Cell, 156(1-2), 146-157.
*These authors contributed equally to this work
Spotlight in TIBS: Popp, M. W.-L. and Maquat L. E. (2014) Defective secretory-protein mRNAs take the RAPP, Trends in Biochemical Sciences, Vol. 39, No. 4, p. 154-156.
Nature Reviews Genetics: Research highlights: Novel mRNA quality control mechanism. Nature Reviews Genetics (2014) vol. 15 (3), p. 144.
The article has been selected as a technical advance by Faculty of 1000.
Karamysheva Z., Diaz-Martinez L.A, Crow S.E, Li B., Yu H. (2009) Multiple Anaphase-promoting Complex/Cyclosome Degrons Mediate the Degradation of Human Sgo1. J. Biol. Chem.,284(3), 1772-80.
Karamysheva Z. N., Surovtseva Y., Vespa L., Shakirov E., Shippen D. E. (2004) A C-terminal Myb-extension domain defines a novel family of double-strand telomeric DNA binding proteins in Arabidopsis. J. Biol. Chem.,279 (46), 47799-47807.
Karamyshev A. L., Karamysheva Z. N., Yamami T., Ito K., Nakamura Y. (2004) Transient idling of posttermination ribosomes ready to reinitiate protein synthesis. Biochimie, 86 (12), p.933-938.
Karamysheva Z., Wang L., Shrode T., Bednenko J., Hurley L. A., Shippen D. E. (2003) Developmentally programmed gene elimination in Euplotes crassus facilitates a switch in the telomerase catalytic subunit. Cell, 113:565-576.
Preview on this paper has been published in Cell (2003) 113:552-554 (Cristofari G, Lingner J. Fingering the ends: how to make new telomeres).
Karamysheva Z. N, Karamyshev A. L., Ito K., Yokogawa T., Nishikawa K., Nakamura Y., Matsufuji S. (2003) Antizyme frameshifting as a functional probe of eukaryotic translational termination. Nucleic Acids Res., 31:5949-5956.
This paper was selected as technical advancebyFaculty 1000.
Ray S., Karamysheva Z., Wang L., Shippen D. E., Price C. M. (2002) Interactions between telomerase and primase
physically link the telomere and chromosome replication machinery.
Mol. Cell. Biol., 22:5859-5868.
Karamyshev A. L., Karamysheva Z. N., Kajava A. V., Ksenzenko V. N., Nesmeyanova M. A. (1998) Processing of Escherichia coli alkaline phosphatase: role of the primary structure of the signal peptide cleavage region. J. Mol. Biol., 277:859-870.
Nesmeyanova M. A., Karamyshev A. L., Karamysheva Z. N., Kalinin A. E., Ksenzenko V. N., Kajava A. V. (1997) Positively charged lysine at the N-terminus of the signal peptide of the Escherichia coli alkaline phosphatase provides the secretion efficiency and is involved in the interaction with anionic phospholipids. FEBS Lett., 403:203-207.