PROTEOMICS

The Proteomics/Metabolomics core facility at the Center for Biotechnology and Genomics provides services to the entire TTU community as well as outside customers. 

We offer analysis of proteins and metabolites from cells, tissues, or other biological samples using cutting-edge instrumentation and methods.

Services Offered:

• Protein IDs by LC-MS/MS and MALDI-TOF/TOF
• Whole proteome profiling from cells, tissue, etc.
• In-gel/in-solution digestion of proteins
• Data analysis using advanced software such as MASCOT, Proteome Discoverer, Compound Discoverer, LipidSearch, and Ingenuity Pathway Analysis (IPA)
• Protein quantification (label-free quantification, tagging)
• Analysis of post-translational modifications in proteins (e.g. phosphorylation)
• Global Metabolite/lipid profiling
• Targeted metabolite analysis (identification and quantification)

Instruments Offered:

• Mass Spectrometers: Q-Exactive HF, LTQ Orbitrap Velos, ABI SCIEX 4800 (MALDI-TOF/TOF)
• LC systems: Dionex-Ultimate 3000 Nano-LC, Vanquish UHPLC, Dionex-Ultimate 3000 with fraction collector for peptide fractionation

The Proteomics/Metabolomics Team

• Dr. Yehia Mechref, CBG Director, Paul W. Horn Distinguished Professor, Robert A. Welch Chair in Chemistry, and Associate Vice President of Research & Innovation
• Dr. Fang Chen, Mass Spectrometry, Proteomics, and Metabolomics Core Manager, Research Professor
• Dr. Songyue Shi, Research Associate
• Most Zakia Ferdous Ara Begum, M.S., Research Associate
• Shannon Sears, M.A., Project Manager

Brochure for Genomics, Proteomics, and Bioinformatics Services Provided at the Center