Dr. Yehia Mechref
Principal Research Interests
- Chemical Separation
The current advances in bioanalytical methods and instrumentation have greatly facilitated the understanding of biological processes at the molecular level. Every living organism in its environment represents a biochemically unique system whose biological functions can be better understood through analytical measurements. Isolated analytical measurements, which may help assessing the state of health within the organisms under study, are now routinely being made. Distinguishing between the “apparently healthy” and “diseased” individuals in clinical chemistry assays is often complicated by the biochemical individuality which has been recognized as an experimental complication of acquiring data from human subjects. Moreover, this aspect commonly results in considerable ranges for both often exist.
The rapidly developing fields of genomics, transcriptomics, proteomics, glycomics, and metabolomics are generating a vast body of biochemical information which is only sorted out through Systems Biology. Systems Biology, here, is referring to the strategy of pursuing integration of complex data about the interactions in biological systems from diverse bioanalytical techniques using multidisciplinary tools and personnel. Many research laboratories have been focusing their efforts on the overwhelming task of characterizing proteins and their voluminous posttranslation modifications following the Human Genome Project and the subsequent efforts to characterize the genomes of model animals. Inquiries into the glycomes and metabolomes of physiological fluids and tissues have been prompted by the unprecedented advances in biomolecular characterization and quantification of proteins. The abality to address these inquiries have been mainly attributed to the methodological/technological advances in biomolecular mass spectrometry and microscale separation science, as well as to the greatly improved computational capabilities (bioinformatics) which is facilitating fast data visualization and interpretation. The overarching activities in my research group are focused on the utility of molecular mass spectrometry to understand biomolecular changes attributing to disease development and progression.
Glycomics and glycoproteomics continue to be highly dynamic and interesting research areas due to the needs to comprehensively understand the biological attributes of glycosylation in many important biological functions such as immune response, cell development, cellular differentiation/adhesion and host-pathogen interactions. Moreover, aberrant glycosylation for decades has been recognized as the attribute of many mammalian diseases, including osteoarthritis, cystic fibrosis and cancer. This being said, glycomic and glycoproteomic analyses remain to be analytically very challenging mainly due to the high complexity and microheterogeneity of glycosylation processes and machineries. Therefore, more cutting-edge-analytical approaches are needed to allow more comprehensive characterization of glycosylation and subsequently better understanding of its biological roles. The ultimate goal of the research activities of my group is the comprehensive characterization of glycoprotein glycosylation sites and microheterogeneity of such sites. Accordingly, we are developing several glycomic and glycoproteomic approaches facilitating the achievement of this ultimate goal. Since interpretation and processing of glycomic and glycoproteomic data is laborious and time consuming, we are also collaborating with bioinformatics experts to develop different bioinformatic tools aiding rapid and automated data interpretation and processing. These research activities are intended to facilitate the analysis of large numbers of biological samples, thus facilitating a better understanding of disease development and progression at a molecular level.
Book Chapter (2 out of 12)
- Y. Mechref in Carlos D Garcia and Emanuel Carrilho (eds.) Fundamental Concepts, Practical Applications, and Limitations of Capillary Electrophoresis and Microchip Capillary Electrophoresis, 2013, John Wiley & Sons, Inc., NJ, 367-383.
- Y. Mechref "Monosaccharide Compositional Analysis of Glycoproteins and Glycolipids: Utility in the diagnosis/prognosis of Diseases", in N. Volpi, (ed.) Capillary Electrophoresis of Carbohydrates. From Monosaccharide to Complex Polysaccharides, 2011, Humana Press, Secaucus, NJ, 237-269.
Review Articles (6 out of 16, # co-corresponding author, * corresponding author)
- #F Kobeissya,T. H. Mouhieddine, A. Nokkari, M. Itani, M. Mouhieddine, Z. Zhang, R. Zhu, M. S. Gold, K. K.Wang, Y. Mechref "Recent updates on drug abuse analyzed by neuroproteomics studies: Cocaine, Methamphetamine and MDMA" Transl. Proteomics, 3 (2014) 38-52.
- *Y. Mechref, Y. Hu, S. Zhou, and H. Tang, "Quantitative Glycomics by Mass Spectrometry and Liquid Chromatography-Mass Spectrometry." Encyclopedia of Analytical Chemistry. (2014)1–22.
- H. Tang, A. Mayampurath, C. Yu and Y Mechref "Bioinformatics Protocols in Glycomics and Glycoproteomics" Current Protocols in Protein Science, 76:2.15 (2014) 1-7.
- *Y. Mechref, Y. Hu, J. L. Desantos-Garcia, and H. Tang, "Quantitative Glycomic Strategies" Mol. Cell. Proteomics, 12 (2013) 874-884.
- *Y. Mechref, Y. Hu, A. Garcia, A. Hussein "Disease Biomarkers through Mass Spectrometry Based Glycomics" Electrophoresis, 33 (2012)1755-67.
- *Y. Mechref "Use of CID/ETD Mass Spectrometry to Analyze Glycopeptides. Current Protocols in Protein Science. 68:12.11 (2012) 1–12.
Research Papers (24 out of 134, #co-corresponding author, *corresponding author, over 30 articles are cancer related)
- *S. Zhou, Y. Hu, J. L. DeSantos-Garcia and Y. Mechref "Reliable Quantification of Permethylated N-Glycans through Multiple Reaction Monitoring (MRM) LC-MSMS" J. Amer. Soci. Mass Spectrom. 26 (2015) 596-603.
- *Y. Hu, S. Zhou, C-Y. Yu, H. Tang and Y. Mechref "Automated Annotation and Quantitation of Glycan by LC-ESI-MS analysis using MultiGlycan-ESI Computational Tool" Rapid Commun. Mass Spectrom., 29 (2015) 1-8.
- W. A. Elmasri, M-E. F. Hegazy, Y. Mechref and P. W. Pare´, "Cytotoxic saponin poliusaposide from Teucrium polium" RSC Adv., 5 (2015) 27126–27133.
- W. A. Elmasri, T. Yang, P. Tran, M-E. F. Hegazy, A. N. Hamood, Y. Mechref, Paul W. Paré "Teucrium polium phenylethanol and iridoid glucoside identification and inhibition of biofilm forming Staphylococcus aureus" J. Natur. Products, 78 (2015) 2-9.
- *E. Song, A. Mayampurath, C-Y. Yu, H. Tang, and Y. Mechref "Glycoproteomics: Identifying the Glycosylation of Prostate Specific Antigen at Normal and High Isoelectric Point by LC-MS/MS." J. Proteome Research, 13 (2014) 5570- 5580.
- *E. Song, R. Zhu, Z. T. Hammoud, Y. Mechref "LC-MS/MS Quantitation of Esophagus Disease Blood Serum Glycoproteins by Enrichment with Hydrazide Chemistry and Lectin Affinity Chromatography." J. Proteome Research, 13 (2014) 4808- 4820.
- T.H. Tsai, M. Wang, C. Di Poto, Y. Hu, S. Zhou, Y. Zhao, R.S. Varghese, Y. Luo, M.G. Tadesse, D.H. Ziada, C.S. Desai, K. Shetty, Y. Mechref, H.W. Ressom "LC-MS Profiling of N-glycans Derived from Human Serum Samples for Biomarker Discovery in Hepatocellular Carcinoma."J. Proteome Research, 13 (2014) 4859- 4868.
- #A. Mayampurath, E. Song, A. Mathur, C.Y. Yu, Z. Hammoud, Y. Mechref, H. Tang "Label-Free Glycopeptide Quantification for Biomarker Discovery in Human Sera." J. Proteome Research, 13 (2014) 4821- 4832.
- A. I. Molosh, P. L. Johnson, J. P. Spence, D. Arendt, L. M. Federici, C. Bernabe, S. P. Janasik1, Z. M. Segu, R. Khanna2, C. Goswami, W. Zhu, S-J. Park, L. Li, Y. Mechref, D. W. Clapp, A. Shekhar "Social learning and amygdala disruptions in Nf1 mice are rescued by blocking p21-activated." Nature Neuroscience, 17 (2014) 1583- 1590.
- W.A. Elmasri, M.E. Hegazy, M. Aziz, Koksal E, W. Amor, Y. Mechref, A.N. Hamood, D.B. Cordes, P.W. Paré "Biofilm blocking sesquiterpenes from Teucrium polium." Phytochemistry, 103 (2014) 107-113.
- #A. Mayampurath, C.Y. Yu, E. Song, J. Balan, Y. Mechref, H. Tang "Computational framework for identification of intact glycopeptides in complex samples." Anal. Chem., 86(1) (2014) 453-463.
- *A. D. Garcia, J. L Chavez and Y. Mechref "RAPID AND SENSITIVE LC-ESI-MS OF GANGLIOSIDES" J. Chromatogr. B, 947-948 (2014) 1-7.
- T-H. Tsai, M. G. Tadesse, C. Di Poto, L. K. Pannell, Y. Mechref, Y. Wang, and H. W. Ressom "Multi-profile Bayesian Alignment Model for LC-MS Data Analysis with Integration of Internal Standards" Bioinformatics, 29 (2013):2774-80.
- F. Wang, C. Chmil, F. Pierce, K. Ganapathy, B. Gump, J. Mackenzie, Y. Mechref, and K. Bendinskas "Immobilized Metal Affinity Chromatography and Human Serum Proteins" J. Chromatogr. B, 934 (2013) 26-33.
- *E. Song and Y. Mechref "LC-MS/MS Identification of the O-Glycosylation and Hydroxylation of Amino Acid Residues of Collagen α-1 (II) chain from Bovine Cartilage" J. Proteome Res., 12 (2013) 3599- 3609.
- Leymarie N, Griffin PJ, Jonscher K, Kolarich D, Orlando R, McComb M, Zaia J, Aguilan J, Alley WR, Altmann F, Ball LE, Basumallick L, Bazemore-Walker CR, Behnken H, Blank MA, Brown KJ, Bunz SC, Cairo CW, Cipollo JF, Daneshfar R, Desaire H, Drake RR, Go EP, Goldman R, Gruber C, Halim A, Hathout Y, Hensbergen PJ, Horn DM, Hurum D, Jabs W, Larson G, Ly M, Mann BF, Marx K, Mechref Y, Meyer B, Möginger U, Neusüβ C, Nilsson J, Novotny MV, Nyalwidhe JO, Packer NH, Pompach P, Reiz B, Resemann A, Rohrer JS, Ruthenbeck A, Sanda M, Schulz JM, Schweiger-Hufnagel U, Sihlbom C, Song E, Staples GO, Suckau D, Tang H, Thaysen-Andersen M, Viner RI, An Y, Valmu L, Wada Y, Watson M, Windwarder M, Whittal R, Wuhrer M, Zhu Y, Zou C. "Interlaboratory study on differential analysis of protein glycosylation by mass spectrometry: the ABRF glycoprotein research multi-institutional study 2012." Mol. Cell Proteomics, 12(2013):2935- 2951.
- *A. D. Garcia, J. L Chavez and Yehia Mechref "LC-MS of Sugar Nucleotides Using High Mass Resolution and Accuracy Instrument" Rapid Commun. Mass Spectrom, 27 (2013) 1794- 1800.
- C-Y. Yu, A. Mayampurath, Y. Hu, Y. Mechref and H. Tang "Automated Annotation and Quantification of Glycans Using Liquid Chromatography Mass Spectrometry (LC-MS)" Bioinformatics, 29 (2013) 1706- 1707.
- *Y. Hu, S. Zhou, S. Khalil, K. Renteria, Y. Mechref "Mass Spectrometry Profiling of Glycans Derived from Mouse Brain Tissue Sections" Anal. Chem., 85 (2013) 4074- 4079.
- *Y. Hu, J. De-Santos-Garcia, Y. Mechref, "Comparative Glycomic Profiling of Isotopically Permethylated N-Glycans by LC-ESI-MS", Rapid Commun. Mass Spectrom., 27 (2013) 865-877.
- J. Burke, J. Chen, G. Burow, Y. Mechref, D. Rosenow, P. Payton, Z. Xin, and C. M. Hayes "Leaf Dhurrin Content is a Quantitative Measure of the Level of Pre- and Post-Flowering Drought Tolerance in Sorghum", Crop Sci., 53 (2013) 1-6.
- *Y. Jmeian, L.A. Hammad, Y. Mechref "Fast and efficient online release of N-glycans from glycoproteins facilitating liquid chromatography−tandem mass spectrometry glycomic profiling" Anal. Chem., 84 (2012) 8790- 8796.
- *Y. Hu and Y. Mechref "Comparing MALDI-MS, LC-MALDI-MS and LC-ESI-MS Glycomic Profiles of Permethylated N-glycans Derived from Model Glycoproteins and Human Blood Serum." Electrophoresis, 33 (2012) 1755-67.
- *E. Song, S. Pyreddy, and Y. Mechref "Quantification of Glycopeptides by Multiple Reaction Monitoring LC-MS/MS" Rapid Commun. Mass Spectrom., 26(2012) 1941-54.