Publications for Akhilesh Shakya, Ph.D.

Research and review articles

2023

AK Shakya, B Mallick, KS Nandakumar. A Perspective on Oral Immunotherapeutic Tools and Strategies for Autoimmune Disorders. Vaccines, 2023;11(6):1031.

AK Shakya, KS Nandakumar. Polymer Chemistry Defines Adjuvant Properties and Determines the Immune Response against the Antigen or Vaccine. Vaccines 2023;11(9):1395.

2022

AK Shakya. Functionalized cryogel monoliths for fast and selective separation of nucleic acids directly from the crude lysate. Biomedical Chromatography, 2022;36:e5333.

LD Nesovic, AK Shakya*, HS Gill. Treating allergies via skin-recent advances in cutaneous allergen immunotherapy. Advanced Drug Delivery Reviews, 2022, 114458. (*Sharing first authorship). 

JJ Landers, KW Janczak, AK Shakya, V Zarnitsyn, SR Patel, JRB Jr, HS Gill, JJ O'Konek. Targeted allergen-specific immunotherapy within the skin improves allergen delivery to induce desensitization to peanuts. Immunotherapy, 2022, 10.2217/imt02021-0226.

2020

AK Shakya, CH Lee, HS Gill. Microneedles mediated allergen-specific immunotherapy for the treatment of airway allergy in mice. Molecular Pharmaceutics, 2020;17:3033-3042. 

2019

AK Shakya, RS Ingrole, G Joshi, MdJ Uddin, S Anvari, CM Davis, HS Gill.                   Microneedles coated with peanut allergen enable desensitization of peanut-sensitized mice. Journal of Controlled Release, 2019;314:38-47. 

2018

AK Shakya, CH Lee, MdJ Uddin, HS Gill. Assessment of Th1/Th2 bias of STING agonists coated on microneedles for possible use in skin allergen immunotherapy. Molecular Pharmaceutics, 2018;15:5437-5443. 

AK Shakya, CH Lee, HS Gill. Coated microneedle-based cutaneous immunotherapy prevents Der p1-induced airway allergy in mice. Journal of Allergy and Clinical Immunology, 2018;142:207-2011.e3. 

AK Shakya, KS Nandakumar. An update on smart biocatalysts for industrial and biomedical applications. Journal of the Royal Society Interface 2018;15:20180062. 

AK Shakya, KS Nandakumar. Antigen-specific tolerization and targeted delivery as therapeutic strategies for autoimmune diseases. Trends in Biotechnology, Cell Press 2018;36:7:686-699. 

2017 

AK Shakya, CH Lee, HS Gill. Coated microneedles for cutaneous preventive immunotherapy against airway allergies. Journal of Controlled Release, 2017;265:75-82. 

AK Shakya, U Kandalam. Three-dimensional macroporous Materials for Craniofacial Bone Tissue Engineering. British Journal of Oral & Maxillofacial Surgery, 2017;55:875-91. 

W Tao, B Hurst, AK Shakya, MdJ Uddin, RS Ingrole, M Hernandez-Sanabria, R Arya, L Bimler, S Paust, EB Tarbet, HS Gill. Consensus M2e peptide conjugated to gold nanoparticles confers protection against H1N1, H3N2, and H5N1 influenza A viruses. Antiviral Research, 2017;141:62-72. 

2016

AK Shakya, W Tao, MYE Chowdhury, HS Gill. Mucosal vaccine delivery: Current state and a pediatric perspective. Journal of Controlled Release, 2016;240:394-413. 

AK Shakya, A Kumar, R Holmdahl, KS Nandakumar. Macrophage-derived reactive oxygen species protect against autoimmune priming with a defined polymeric adjuvant. Immunology, 2016;147:125-32. 

2015

AK Shakya*, A Srivastava, A Kumar. Polymeric cryogel-based boronate affinity chromatography for separation of Ribose Nucleic Acid. Wiley Current Protocols in Nucleic acid chemistry, 2015;63;10:1-10. 

AK Shakya, HS Gill. A comparative study of microneedle-based cutaneous immunization with other conventional routes to assess the feasibility of microneedles for allergy immunotherapy. Vaccine, 2015;33:4060-4. 

E Jain, A Damania, AK Shakya, A Kumar, SK Sarin, A Kumar. Fabrication of macroporous cryogels as potential hepatocyte carriers for bioartificial liver support. Colloids Surf B Biointerfaces, 2015;136:761-71. 

2014

AK Shakya, KS Nandakumar, A Kumar. Chemical cross-linking abrogates the adjuvant potential of natural polymers. Royal Society of Chemistry Advances, 2014;4:13817-21. 

AK Shakya, R Holmdahl, KS Nandakumar, A Kumar. Polymeric cryogels are biocompatible and their biodegradation is independent of oxidative radicals. Journal of Biomedical Materials Research Part A, 2014;102:3409-18. 

AK Shakya, KS Nandakumar. A synthetic polymer as an adjuvant in collagen-induced arthritis. Wiley Current protocols Mouse biology, 2014;4:1-12. 

2013

AK Shakya, R Holmdahl, KS Nandakumar, A Kumar. Characterization of chemically defined poly-N-isopropyl acrylamide-based copolymeric adjuvants. Vaccine, 2013;31:3519-27. 

AK Shakya, A Kumar. Recent developments in atom transfer radical polymerization initiators for the development of polymer-protein bioconjugates. Journal of Bioscience and Biotechnology, 2013;2:1-11. (Invited article) 

AK Shakya, KS Nandakumar. Polymeric adjuvants in studying autoimmune responses and vaccination against infectious diseases. Journal of the Royal Society Interface, 2013; 10:20120536. 

2012

AK Shakya, KS Nandakumar. Polymers as immunological adjuvants: An update on recent developments. Journal of Bioscience and Biotechnology, 2012;1:199-210. (Invited article)

A Srivastava, AK Shakya*, A Kumar. Boronate affinity chromatography of cells and biomacromolecules using cryogels. Enzyme and Microbial Technology, 2012;51:373-81(*Sharing first authorship). 

2011

AK Shakya, A Kumar, D Klaczkowska, M Hultqvist, K Hagenow, R Holmdahl, KS Nandakumar. Collagen Type II and a Thermo-Responsive Polymer of N-Isopropylacrylamide Induce Arthritis Independent of Toll-Like Receptors: A Strong Influence by Major Histocompatibility Complex Class II and Ncf1 Genes. American Journal of Pathology, 2011;5:2490-2500. 

AK Shakya, A Kumar, and KS Nandakumar. A thermo-responsive polymeric [poly(N-isopropyl acrylamide)] adjuvant with collagen-induced autoimmunity and arthritis in mice. Journal of the Royal Society Interface 2011; 8:1748-59. 

2010

AK Shakya, P Sharma, A Kumar. Synthesis and characterization of thermo-responsive poly(N-isopropyl acrylamide)-bovine liver catalase bioconjugate. Enzyme and Microbial Technology, 2010;47:277-82. 

AK Shakya, H Sami, A Srivastava, A Kumar. Stability of responsive polymer–protein bioconjugates. Progress in Polymer Science, 2010;35:459-86. 

 

Book chapters

 
1.    AK Shakya, KS Nandakumar. Nano-size-based drug delivery systems for autoimmune diseases. In: Autoimmune disorders: Risk factors, pathogenesis, and treatments. Edited by KS Nandakumar. Nova Science publisher 2019 (ISBN:9781536160468).

 

2.    AK Shakya, KS Nandakumar. Nanobiocatalysts for industrial applications. In: Characterization and biology of nanomaterials for drug delivery. Edited by S Mohapatra, S Ranjan, N Dasgupta, R Mishra, and S Thomas. Elsevier 2018. (ISBN:9780128140314).

 

3.    AK Shakya, KS Nandakumar. Biomaterials for induction and treatment of autoimmunity. In: Advances in biomaterials for biomedical applications. Edited by A Tripathi, and JS Melo. Springer Singapore 2017. (ISBN:9789811033278).

 

4.    AK Shakya, A Kumar. Biocompatibility of macroporous cryogel materials, In Supermacroporous cryogels: Biomedical and biotechnological applications. Edited by A Kumar, CRC Press 2015. (ISBN:9781482228816).

 

5.    AK Shakya, H Madhyastha. Polymeric nanoparticles for vaccine delivery, In Integrating biologically-inspired nanotechnology into medical practice. Edited by A Nanda, and MA Bhat, IGI Global 2015. (ISBN13:9781522506102).

 

6.    AK Shakya, KS Nandakumar. Stimuli-responsive polymers as adjuvants and carriers for antigen delivery. In: Responsive materials and methods. Edited by A Tiwari, and H Kobayashi. Scrivener Publishing Wiley 2013. (ISBN:9781118686225).

 

7.    AK Shakya, KS Nandakumar. Nanomaterials applications in activation and suppression of immune response. In: Handbook of research diverse application of nanotechnology in biomedicine, chemistry, and engineering. Edited by S Soni, and A Salhotra. IGI Global 2014. (ISBN13:9781466663633).

 

8.    A Srivastava, AK Shakya, A Kumar. Particulate/cell separations using macroporous monolithic matrices. In: Advanced separations by specialized sorbents. Edited by ES Dragan, CRC Press 2014. (ISBN:9781482220551).

 

9.    AK Shakya, KS Nandakumar. New generation vaccines: the need for safe and improved adjuvant. In: Vaccines and vaccine technology. Edited by JR Vasconcelos OMICS Group eBooks 2013, Foster City, USA. (ISBN:9781632780423)

 

Patents

1.    Patent (USA patent No. US20200138941A1): Methods and Devices for the Treatment of Food Allergies, HS Gill, AK Shakya (Texas Tech University). Status: GRANTED. https://patents.google.com/patent/US20200138941A1/en

 

2.    Patent (USA patent No. WO2019070631A1): Pollen grains for treatment of airway and other allergies, HS Gill, AK Shakya (Texas Tech University). Status: published. https://patents.google.com/patent/WO2019070631A1/en

 

3.    Patent (TTU Serial No. 43438611): Targeting Junctional Epithelium in the Gingival Crevice for Immune Modulation, HS Gill, Rohan SJ Ingrole, AK Shakya (Texas Tech University). Status: filed. https://ttu.flintbox.com/technologies/dc5bfb56-017c-480d-8657-fe7a4804a9e3

 

4.     Patent (TTU Serial No. 63/595,130): Methods and Protocols to Develop Food-Induced Allergic and Anaphylactic Responses in a Porcine Animal Model, HS Gill, AK Shakya, Brittany Backus, Lazar Nesovic. (Texas Tech University). Status: filed in 2023. 

 

Research Grants & Awards

2022

AK Shakya (Lead PI), NIH R21. Pollen grains for peanut allergy immunotherapy, 0.3 million USD, Declined.

AK Shakya (Lead PI), NIH R21. Pollen grains for airway allergy treatment, 0.3 million USD, Declined.

AK Shakya (Lead PI), IHR21. Pollen grains for egg allergy treatment, 0.3 million USD, Declined.

2021

AK Shakya (Lead PI), Arthritis National Research Foundation (ANRF), Microneedle mediated cutaneous immunotherapy for rheumatoid arthritis, 99K USD, Declined.

AK Shakya, (Lead PI), NIH R21, Microneedle-mediated cutaneous allergen immunotherapy for desensitization of milk allergy, 0.3 million USD, Declined.

2018   

HS Gill (Lead PI), AK Shakya (co-PI), NIH-R01, Microneedles for treatment of peanut allergy, 3.4 million USD, Awarded.

HS Gill (Lead PI), AK Shakya (co-PI), NIH STTR, Microneedle based tragated allergen specific immunotherapy for treatment of peanut allergies, 0.9 million, USD, Awarded.

 2016

HS Gill (Lead PI), AK Shakya (co-PI), NIH R01, Direct HIV vaccine delivery into oral cavity mucosa using microneedles to trigger mucosal and systemic immunity. 2.4 million, USD, Declined.