Dr. Joachim Weber
Title: Associate Professor and Associate Chair
Education: Ph.D., Medical University of Lübeck, Germany, 1990
Postdoctoral, University of Rochester, NY, 1990-1995
Research Assistant Professor, University of Rochester, NY, 1995-2003
Research Area: Biochemistry
Office: Chemistry 232-D
Webpage: Research Group
Principal Research Interests
- ATP Synthase – The World's Smallest Rotary Motor
- Mutational Analysis of Enzymatic Function
- Biophysical Chemistry
Dr. Weber's current work focuses on the question how ATP binding and hydrolysis in the catalytic site drive subunit rotation, using a variety of approaches. The applied techniques range from molecular biology (site-directed mutagenesis) to biochemistry (protein chemistry, enzyme kinetics) to biophysical chemistry (fluorescence spectroscopy) and molecular modeling. Molecular dynamics simulations and torque measurements by single-molecule analysis are performed in collaboration with other laboratories. Noteworthy recent results of this research were (a) the identification of the catalytically-active nucleotide binding site, (b) the functional analysis of a unique hydrogen-bonding network between two subunits of the enzyme, and (c) further insight into the coupling mechanism between catalysis and rotation.
Research in Dr. Weber's lab is supported by the NIH.
- Kolawole, O., Millikan, C., Kumar, M., Ispas, I., and Weber, J. (2022) Microbial induced mechano-petrophysical modified properties to improve hydrocarbon recovery in carbonate reservoirs. Geomech. Energy Environ. https://doi.org/10.1016/j.gete.2022.100399.
- Kolawole, O., Millikan, C., Kumar, M., Ispas, I., Schwartz, B., Weber, J., Badurina, L., and Segvic, B. (2022) Impact of microbial-rock-CO2 interactions on containment and storage security of supercritical CO2 in carbonates. Int. J. Greenh. Gas Control 120, 103755.
- Avila-Barrientos, L.P., Cofas-Vargas, L.F., Agüero-Chapin, G., Hernández-García, E., Ruiz-Carmona, S., Valdez-Cruz, N.A., Trujillo-Roldán, M., Weber, J., Ruiz-Blanco, Y.B., Barril, X., and Garcia-Hernandez, E. (2022) Computational design of inhibitors targeting the catalytic subunit of Escherichia coli FoF1-ATP synthase. Antibiotics 11, 557.
- Mnatsakanyan, N., Park, H., Wu, J., He, X., Llaguno, M.C., Latta, M., Miranda, P., Murtishi, B., Graham, M., Weber, J., Levy, R.J., Pavlov, E.V., and Jonas, E.A. (2022) Mitochondrial ATP synthase c-subunit leak channel triggers cell death upon loss of its F1 subcomplex. Cell Death Differ. https://doi.org/10.1038/s41418-022-00972-7.
- Li, Y., Valdez, N.A., Mnatsakanyan, N., and Weber, J. (2021) The nucleotide binding affinities of two critical conformations of Escherichia coli ATP synthase. Arch. Biochem. Biophys. 707, 108899.
- Kolawole, O., Ispas, I., Kumar, M., Weber, J., and Zhao, B. (2021) Time-lapse biogeomechanical modified properties of ultra-low permeability reservoirs. Rock Mech. Rock Eng. 54, 2615 – 2641.
- Kolawole, O., Ispas, I., Kumar, M., Weber, J., Zhao, B., and Zanoni, G. (2021) How can biogeochemical alterations in shales impact caprock integrity and CO2 storage? Fuel 291, 120149.
- Swartz, D.J., Singh, A., Sok, N., Thomas, J.N., Weber, J., and Urbatsch, I.L. (2020) Replacing the eleven native tryptophans by directed evolution produces an active P-glycoprotein with site-specific, non-conservative substitutions. Sci. Rep. 10, 3224
- Mnatsakanyan, N., Llaguno, M.C., Yang, Y., Yan, Y., Weber, J., Sigworth, F.J., and Jonas, E.A. (2019) A mitochondrial megachannel resides in monomeric F1Fo ATP synthase. Nat. Commun. 10, 5823.
- Li, Y. Ma, X., and Weber, J. (2019) Interaction between γC87 and γR242 residues participates in energy coupling between catalysis and proton translocation in Escherichia coli ATP synthase. Biochim. Biophys. Acta 1860, 679 - 687.
- Mnatsakanyan, N., Li, Y., and Weber, J. (2019) Identification of two segments of the γ subunit of ATP synthase responsible for the different affinities of the catalytic nucleotide binding sites. J. Biol. Chem. 294, 1152 - 1160.
Department of Chemistry & Biochemistry
Address1204 Boston Avenue, Lubbock, TX 79409-1061