Autoclaving Biological Waste
- Waste is to be collected in leak-proof containers. Bags with the universal biohazard symbol must be used to collect solid waste. If you steam-sterilize your waste, make sure your bags are autoclavable.
- Other than benchtop collection, biohazard bags must be contained in a solid, leak-proof container with a lid capable of decontamination. The container must be marked with the biohazard symbol.
Preparing for the Autoclave
- Prepare waste to allow steam circulation (i.e., loosely tie bags, loosen lids or loosely cover with foil). Apply autoclave tape.
- If you have to transport waste through common areas, use a closable tub w/ locking lid; take the autoclave tub(s) with you.
- At the autoclave, place waste in a leak-proof, autoclavable tub. Do not overload the tub.
Autoclaving and Final Disposal
- Autoclave waste no less than 30 minutes at 121°C and 15psi pressure.
- After autoclaving, Record the run in the log, carefully remove the load, and affix a "treated" sticker to biobags.
- Place the biobag in a black trash bag.
- Lab personnel can then discard waste in the dumpster.
- Log the treatment on your Treatment Log.